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Testing for tocopherols and tocotrienols (collectively known as tocols,) amphipathic and lipid-soluble compounds that are easily oxidized when subjected to heat, light and alkaline conditions.

Tocopherols and Tocotrienols

Qualitative and quantitative analysis of Vitamin E and its various forms. Alpha-Tocopherol, Beta-Tocopherol, Delta-Tocopherol, Gamma-Tocopherol, Vit-E Acetate, Vit-E Succinate, Alpha-Tocotrienols, Beta-Tocotrienols, Delta-Tocotrienols and Gamma-Tocotrienols.

Method Summary

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Gas chromatography offers qualitative and quantitative analysis of Vitamin E and its various forms, collectively referred to as Tocopherols and Tocotrienols. The aim of sample preparation is to isolate the Tocopherol/Tocotrienol fraction and to convert all conjugated or esterified products to prepare for GC analysis. Vitamin E and its various isoforms are extracted in the presence of an internal standard and are then concentrated in the unsaponifiable fraction.

After saponification, the unsaponifiable material including the Tocopherols/Tocotrienol portion is extracted using a nonpolar solvent.

Lastly, derivatization is performed using a basic sialylation procedure. The resulting Tocopherols/Tocotrienols are then analyzed on an Agilent GC-FID chromatograph against the internal standard.

Reference: In-House Method
Analysis Platform: Agilent 6890, 7890 GC-FID
Data Reported: The amount of tocopherol / tocotrienol in mg/gram, % by weight
Analytical Targets:
  • Alpha-Tocopherol
  • Beta-Tocopherol
  • Delta-Tocopherol
  • Gamma-Tocopherol
  • Vit-E Acetate
  • Vit-E Succinate
  • Alpha-Tocotrienols
  • Beta-Tocotrienols
  • Delta-Tocotrienols
  • Gamma-Tocotrienols
Sample Requirements: Please contact us with regards to your specific sample type.

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