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Sterols & Stanols
Gas chromatography testing to measure cholesterol, cortisol (and other corticosteroids), ergosterol, campesterol, sitosterol, and stigmasterol.

Sterols and Stanols

Thorough testing of biological material, nutraceutical oils, industrially-produced food products and more for phytosterols (plant sterols), zoosterols (animal sterols) and mycosterols (fungal sterols).

Method Summary

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Gas chromatography offers qualitative and quantitative analysis of sterols and stanols. The aim of sample preparation is to isolate the sterol fraction and to convert all conjugated or esterified sterols into free phytosterols for GC analysis. Lipids (containing sterols) are extracted in the presence of an internal standard. The sterols are then concentrated in the unsaponifiable fraction.

After saponification, the unsaponifiable material including the sterol portion is extracted using a nonpolar solvent. Lastly, derivatization of sterols is performed using a basic sialylation procedure. The resulting sterols are then analyzed on an Agilent GC-FID chromatograph against the internal standard.

Reference: Modified AOCS Official Method / AOAC 994.10
Analysis Platform: Agilent 6890, 7890 GC-FID
Data Reported: The amount of sterol/stanol in mg/gram, % by weight
Analytical Targets:
  • Cholesterol
  • Brassicasterol
  • Stigmasterol
  • Beta-Sitosterol
  • Campesterol
  • Ergosterol
  • 5’Avenasterol
  • And Other Sterols
Sample Requirements: Please contact us with regards to your specific sample type.

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