Phospholipids

A breakdown of phospholipids including phosphatidic acid (PA), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylglycerol (PG) and sphingomyelin (SM).

Method Summary

Phospholipids (PL) are extracted from samples according to published methods for efficient lipid extractions in the presence of internal standards. Lipid extracts are applied to thin-layer plates coated with silica gel. Bands are identified using long-wave UV by RF comparison to appropriate standards based on authentic standardized fat mixtures. Bands of interest are removed and subjected to transmethylation to produce the fatty acid methyl esters. The resulting fatty acid methyl esters are then analyzed on an Agilent 7890B gas-liquid chromatograph. The resulting data for each fatty acid or lipid is reported in ug/g and/or % by weight or as requested.

We can also provide analyses on the amounts of individual phospholipid types (and their fatty acid profiles).

Reference:	In-House Method
Analysis Platform:	GC-FID
Data Reported:	The total amount of phospholipid in μg/g or % by weight
Analytical Targets:	Total Phospholipids or Individual Phospholipids Including: Phosphatidylcholine (PC) Phosphatidylethanolamine (PE) Phosphatidylinositol (PI) Phosphatidylserine (PS) Phosphatidic Acid (PA) Sphingomyelin (SM)
Sample Requirements:	Please contact us with regards to your specific sample type.