Neutral Lipid Analysis

Testing of edible oils, nutraceuticals oils, food products, biological materials, animal fats and more for triglycerides, triglycerides, steryl and wax esters, diglycerides and monoglycerides.

Method Summary

Lipids (Monoglycerides, Diglycerides, Triglycerides, Free Fatty Acids, Ethyl Esters) are extracted from samples in the presence of an internal standard. Extracts are then separated by solid-phase extraction on silica gel. The bands of interest are identified by RF comparison to appropriate standards by UV light.

Bands of interest are removed and are transmethylated. The resulting fatty acid methyl esters are then analyzed on an Agilent 6890/7890 GC-FID. Data is reported in mg/g and % by weight.

Reference:	In-House
Analysis Platform:	Agilent 6890, 7890 GC-FID
Data Reported:	The amount of total and/or individual class of neutral lipids in mg/gram, % by weight
Analytical Targets:	Monoglycerides Diglycerides Triglycerides Free Fatty Acids Ethyl Esters
Sample Requirements:	Please contact us with regards to your specific sample type.