Phospholipid Analysis

Soy Beans and Chicken Egg

Phophspholipids: are a major component of biological cell membranes working to form membrane bilayers; the sheath like structures formed by the assemblage of proteins, carbohydrates, and various lipids. Biological membranes of cells are usually composed of amphiphilic phospholipids that share common structural components including: a polar organic molecule (e.g. choline, serine), a phosphate group, a glycerol molecule (sometimes replaced by sphingosine as in sphingomyelin) and two long-chain fatty acids (may be saturated or unsaturated of varying chain length and degree of unsaturation). This heterogeneity gives rise to a diverse family of phospholipid structures with differing physico-chemical structures and functions.

Analytical Targets - Phosphatidylcholine (PC), Phosphatidylethanolamine (PE), Phosphatidylinositol (PI), Phosphatidylserine (PS), Phosphatidylglycerol (PG), Sphingomyelin (SM), or Total Phospholipids

Phospholipid Sources - Common sources of industrially produced phospholipids are soya, rapeseed, sunflower, chicken eggs, bovine milk, fish eggs etc. Each source has a unique profile of individual phospholipids species and consequently differing applications in food, nutrition, pharmaceuticals, cosmetics and drug delivery.

Analysis Platform - GC-FID

Method Summary

Phospholipids (PL) are extracted from samples according to published methods for efficient lipid extractions using chloroform/methanol solvent mixtures in the presence of internal standards. Subsequent processing employs methods as described and published in peer-reviewed scientific journals. Lipid extracts are then obtained by solid-phase extraction and the concentrated lipid mixtures are applied to thin-layer plates coated with silica gel. The thin - layer chromatography plate is developed in the appropriate mobile phase for separating the different lipid types. The plate is then dried and sprayed with ANS in order to identify the lipid bands of interest. Bands are identified by Rf comparison to appropriate standards using long - wave UV. Bands of interest are removed and subjected to transmethylation to produce the fatty acid methyl esters. The resulting fatty acid methyl esters are then analyzed on an Agilent 7890B gas-liquid chromatograph with a 60-m DB-23 capillary column (0.32 mm internal diameter) and the separated fatty acids are identified based on authentic standardized fatty acid mixtures of known compositions . The resulting data for each fatty acid or lipid is reported in ug/g and/or % by weight or as requested.

Sample Requirement

  • Liquid Medium – Oils, Biological Cells (RBC / WBC / Serum)– minimum 2.5g equivalency.
  • Tissues/foods/powders – Grains, powders, foods – minimum of 2.5g.
  • Please contact with regards to your specific medium.

Data Reporting

  • CoA (pdf.) provided for sample analysis.
  • Data reported as: μg/g and % by weight for either total phospholipids or by individual phospholipids species.
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